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As an important research tool, RNA is widely used in gene function analysis, development of novel therapeutic strategies and other research applications. We provide high-quality customized RNA synthesis services to meet the different needs of our customers, including general RNA synthesis, RNA modification and labeling, chimeric RNA, 2'-OMe-RNA and other antisense RNAs synthesis, etc. To ensure the high quality of RNA synthesis, we strictly carry out QC testing standards through MS, HPLC, and other methods. In order to ensure the high quality of RNA, the synthesized RNA is strictly implemented QC testing standards, through MS, HPLC and other testing methods to ensure that the final products delivered to customers are high quality RNAs.

siRNA

siRNA is a 19~25nt double-stranded RNA small molecule, which꧙ is a conventional tool used 🎃for gene silencing with low cost and high reproducibility, and has been widely used in biomedical research and clinical drug development. GENERAY Biotechnology can provide customers with high-quality, highly reproducible and customized siRNA services for preclinical studies in cells and animals. All of our siRNA products are purified by HPLC to completely remove the unpaired single strand, and customers only need to dil🎃ute them with the buffer in the box for subsequent experiments.

1.1  Single siRNA customization

Conventional siRNA (15-25bp double-stranded RNA, suitable for general cellular♑ experiments)

Chemically modified siRNA (applicable to animal experiments, siRNA stability and silencing efficiency are enha꧑nced, and the duration of gene silencing is extended)

1.2  siRNA package

Conventional siRNA pack𝓡age (3 for 1 package, ensuring at least one pair of 🐠siRNA knockdown efficiency of 70% or more, suitable for general cell experiments)

♍Chemically modified siRNA package (suitable for animal experime🤡nts, siRNA stability and silencing efficiency are enhanced, and the duration of gene silencing is extended)

miRNA

Generay Biotechnology miRNA products mainly include miRNA Mimics (dꦡouble-stranded annealing products), miRNA Mimic negative control, miRNA inhibito (r RNA single-stranded), and miRNA inhibitor negative꧃ control products. Our miRNA products are manufactured under the ISO9001:2015 quality standard and have been optimized and rigorously tested, with highly stable quality.

2.1 &nbꦓsp;Common miRNA s🔜ynthesis price list (15-25bp) double-stranded/single-stranded

2.2  Modified miRNA synthesis price list (15-25bp) double-stra༺nded/single-stranded

sgRNA and single-stranded RNA

CRISPR/Cas9 has been widely used in Knock out (KO), Knock in (KI), Repression / Activation (CRISPRi / CRISP🌟Ra) and other experiments. As a leading company in gene synthesis, Shanghai GENERAY Biotechnology provides sgRNA design and synthesis, CRISPR/Cas9 plasmid construction (⛦three guarantees and one package). We will provide the following services to assist the development of gene editing drugs and related R&D projects.

gRNA and CRISPR/Cas9 plasmids

Conventional⛦ CRISPR plasmids: pSpCas9(BB)-2A-GFP (abbreviation: PX458, GFP tag), pSpCas9(BB)-2A-Puro (abbreviation: PX459, Puro tag) V2.0 Conventional lentiviral plasmid: lentiCRISPR v2 (Puro tag)

Large-scale RNA synthesis

Large-scale RNA oligonuc🔯leotide synthesis ranges from 50 mg to several grams. To ensure the highest quality RNA, all large-scale RNA oligonucleotide synthesis is processed and purified in an RNase-free environment. All large-scale RNA oligonucleotides were analyzed by HPLC, PAGE, and ESI-MS mass spectrometry.

Service Features

✓ 50 mg to several grams. Length 5-50 nt.

✓ Phosphorothioate (PS), phosphodiestཧer (PO) or PO🗹/PS backbone.

✓ Chimeric oligonucleotides mixed with 2'-OMe, 2'-F-RNA, MOE, LNA or DNA. various oligonucleotide modifications: dyes, spacer groups, junctions.

✓ Multiple purification options av꧙ailable, desalting or HPLC purification, etc.

✓ Multiple chemistry needs such as sterilization/filtration, endotoxin testing, Naဣ+ salt exchange, etc. can be selected. Quality ඣcontrol by HPLC, PAGE, ESI mass spectrometry.

✓ Preferent𒅌ial pricing is available through supply agreements.

Oligos and other couplers

Generay Biotechnology can provide PEG, Peptide, chelating agent (NOTA, DOTA, MAG, DTPA), etc., and other small mꦓolecules coupling service with Oligo.

Quality Assurance

✓ Complete order service system

✓ All RNA oligonucleotides are🌃 produced under strict SOP quality control process

✓ High resoluﷺtion Waters chro꧋matography with ESI-MS to ensure purity

Delivery results

✓ Synthetic RNA lyophilized powder

✓ COA documentation

✓ Assay report (MS & HPLC)

Transfection Reagent

Transfection (i.e., delivery) of genes, RNA, or proteins into cells is a foundational technique in the life sciences. Successful transfection is critical for a range of applications, including studies of gene expression, gene knockdown (i.e., RNAi), genome editing, and protein expressio🔯n for biopharmaceuticals.

Thermo Fisher Scientific is a trusted supplier of pr💎oducts for highly effective cell transfection. The family of lipid-based Invitrogen Lipofectamine transfection reagents is the most cited line of transfection reagents in the world, with a wide range of products designed for highly effective and efficient nucleic acid delivery into a multitude of cell types.As a Thermo Fisher authorized supplier, we can provide official and genuine Lipofectamine series transfection reagents for intracellular delivery oܫf DNA, RNA and proteins.

1. Invitrogen™ Lip🦩ofectamine™ 2000 Transfection Reagent

꧒Strong performanc🐽e delivering DNA in a broad range of workhorse cell lines.

• The most cited DNA r♈eagent in scientific literature

• Strong performance with celꦐl lines that are easy to transfect and those of medium difficulty to transfect

Request a demo at thermofisher.com/2000

2. Invitrogen™ Lipofect𝓡amine™ 3000 Transfection Reagent

Designed for exceptional DNA delivery efficiency into hard-to-transfect, primary𝔉, and cancer cell lines.

• A customizable formulation that is easily optimizable for the exact needs of specific cell types eꦉnabled by the

combination of Lipofectamine 3000 reagent and the Invitrogen™ P3000™ 🐻Enhancer Reagent

• Requires a smaller volume of reagent per reaction, and eliminates the need for multiple transfection experiments, which can result in a 10-fold higher efficiency for difficul🌄t cells

Request a demo at thermofisher.com/3000

3. Invitroge𝕴n™ Lipofect🐓amine™ RNAiMAX Transfection Reagent

The first liꩲpid-mediated reagent for siRNA delivery.

• Effectively provides maximum knockdown with less siRNA and low cytotoxi🌼city

• Works versatilely across a wide range🍎 of cell type🍬s

• Ideal for high-throughput siRNA transfections

Request a demo at thermofisher.com/rnaimax

Transfection Reagent Quotation

Contact Information

●   By Email: rna@syouba.com

●   By Phone: 86-21-67840801/0802